Inverted Microscopy:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Microscopy:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Clinical Proteomics:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Convection:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Hybridization:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Microarray:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Sterility:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Hood:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Cell Culture:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
Marker:Article Title: Screening individual hybridomas by microengraving to discover monoclonal antibodies
Article Snippet: .. .Sucrose (EMD Omnipur, cat. no. 8510) or D-(+)-trehalose dehydrate (Tokyo Chemical Industry Co., cat. no. T0331) .Mineral oil (Sigma-Aldrich, cat. no. M5904) .Glass capillaries (Narishige, cat. no. GC-1) EQUIPMENT .Micromanipulator (Narishige IM-9A, Narishige) mounted on an inverted microscope .Microscope stage insert for 96-well plate .Plasma cleaner (Harrick PDC-32G, Harrick) .Vacuum pump (e.g., Edwards RV-12, Edwards, or Varian DS-102, Varian) .Convection oven (e.g., Precision STC-80, Precision) .Hybridization chamber (Die-Tech, cat. no. DT-1001), replace screws with those from DT-2002 (Die-Tech) .LifterSlips, 25 60 mm (Erie Scientific Company, cat. no. 2-4789) .Micro-fabricated silicon wafers for masters (see Experimental design) .Microcentrifuge with maximum speed of B20,800g (e.g., Eppendorf 5810, Eppendorf) .Benchtop centrifuge (e.g., VWR Galaxy, VWR or Eppendorf) .Microarray centrifuge (e.g., VWR Galaxy MiniArray Microcentrifuge, VWR) .Microarray scanner (e.g., GenePix 4000B or 4200 Autoloader, GenePix) .Bench-top shaker, orbital or lateral (e.g., Labnet Reciprocal 30, Labnet) .Vortex mixer (e.g., VWR, cat. no. 58816-121) .Vacuum desiccators (e.g., Bel-Art) .Bright-field inverted microscope (e.g., Nikon TMS, Nikon) .Hemacytometer (e.g., Hausser Scientific) .Sterile filter tips, 10 ml (VWR, cat. no. 89003-046), 40 ml (VWR, cat. no. 89003-048), 200 ml (VWR, cat. no. 89003-056) and 1,000 ml (VWR, cat. no. 89003-060) .Biosafety cabinet for cell culture .SuperEpoxy2 glass slides (ArrayIt, cat. no. SME2) or 3¢¢ 1¢¢ glass slides (Corning, cat. no. 2947-3x1) coated with poly-lysine according to J. DeRisi’s protocol (http://cat.ucsf.edu/pdfs/PolylysineSlides.pdf) .96-Well plate, flat or round-bottom (e.g., Costar, BD Falcon) .24-Well plate (e.g., BD Falcon, cat. no. 353935) .Four-well plate (Nalge Nunc, cat. no. 267061) .Culture flasks, T25 (e.g., BD Falcon) .Petri dishes, 100 15 mm (BD Falcon, cat. no. 351029), 150 15 mm (BD Falcon, cat. no. 351058) .Sterile microcentrifuge tubes, 1.5 ml (e.g., VWR, cat. no. 89000-028) .Cryovials, 2 ml (Corning, cat. no. 430488) .Sterile conical tubes, 15 and 50 ml (e.g., BD Falcon) .Fine forceps .Scalpels for cutting PDMS .Disposable plastic knives and cups (16 oz) for mixing PDMS .Frosted Scotch Tape .Ultra-fine permanent marker .Kimwipes (Kimberly-Clark) .Bunsen burner or plumber’s propane torch REAGENT SETUP PBS solution (PBS) Prepare a 10 stock containing 9 mM KH2PO4, 100 mM Na2HPO4 and 1.5 M NaCl, and dilute stock as needed to 1 with deionized water. ..
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